Review



tgn46  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Proteintech tgn46
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Tgn46, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pmc13003054-55-44-48
    Average 94 stars, based on 92 article reviews
    tgn46 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice"

    Article Title: ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-026-06125-0

    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and TGN46 (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Figure Legend Snippet: ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and TGN46 (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm

    Techniques Used: Recombinant, Immunoprecipitation, Western Blot, Control, Staining, Marker

    Related Articles

    other:

    Article Title: Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma.
    Article Snippet: The following antibodies for treatment were used: 4 nature portfolio | reporting sum m ary April 2023 anti-CTLA-4 (BE0164, BioXCell), anti-CD8α (BE0061, BioXCell).

    Article Title:
    Article Snippet: The following antibodies for IF/IHC analysis were used: Granzyme B 1:200 (ab4059, Abcam), Rab7B 1:200 (ab193360, Abcam), CD3 1:200 (ab16669, Abcam), PD-L1 1:200 (66248-1-Ig, Proteintech), TGN46 1:200 (13573-1-AP, Proteintech), CD8α 1:200 (ab217344, Abcam), Rab11B 1:100 (15903-1-AP, Proteintech), GRP94 1:200 (14700-1-AP, Proteintech), Secondary antibodies and DAPI: CY3 goat anti-rabbit IgG (GB21303, Servicebio), FITC goat anti-rabbit IgG (GB22303, Servicebio), DAPI (G1012, Servicebio), CY5 goat anti-rabbit IgG (GB27303, Servicebio).

    Western Blot:

    Article Title: The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection
    Article Snippet: PS1 , Rabbit , 5643S; Cell Signaling , WB. .. TGN46 , Rabbit , 13573-1-AP; Proteintech , WB. .. GM130 , Rabbit , ab52649; Abcam , WB, IF, PLA, and IP.



    Similar Products

    tgn46  (Proteintech)
    94
    Proteintech tgn46
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Tgn46, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pmc13003054-55-44-48
    Average 94 stars, based on 1 article reviews
    tgn46 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    coralite594 conjugated tgn46 polyclonal antibody  (Proteintech)
    94
    Proteintech coralite594 conjugated tgn46 polyclonal antibody
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Coralite594 Conjugated Tgn46 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pm41449740-442-23-27
    Average 94 stars, based on 1 article reviews
    coralite594 conjugated tgn46 polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    mouse anti tgn46  (Proteintech)
    94
    Proteintech mouse anti tgn46
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Mouse Anti Tgn46, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pm40902878-95-21-25
    Average 94 stars, based on 1 article reviews
    mouse anti tgn46 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    13573 1 ap  (Proteintech)
    94
    Proteintech 13573 1 ap
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    13573 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pmc12542944-13-4-5
    Average 94 stars, based on 1 article reviews
    13573 1 ap - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti tgn46  (Proteintech)
    94
    Proteintech anti tgn46
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Anti Tgn46, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pm41093223-165-44-45
    Average 94 stars, based on 1 article reviews
    anti tgn46 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    tgn46 monoclonal 66477 1 ig antibodies  (Proteintech)
    94
    Proteintech tgn46 monoclonal 66477 1 ig antibodies
    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and <t>TGN46</t> (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm
    Tgn46 Monoclonal 66477 1 Ig Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13573+1+ap/TGN46+Antibody/pmc12541430__pnas%2E2514150122%2Esapp-11-5-12
    Average 94 stars, based on 1 article reviews
    tgn46 monoclonal 66477 1 ig antibodies - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and TGN46 (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice

    doi: 10.1007/s00018-026-06125-0

    Figure Lengend Snippet: ELAPOR1 was required for recruitment of clathrin onto the pro-AVs. ( A ) Schematic diagrams of flag tagged recombinant ELAPOR1. ( B ) Representative co-immunoprecipitation of recombinant ELAPOR1 with clathrin, AP1, AP2 and AP3 in HEK-293T cells. Western blot analysis showed the interaction between ELAPOR1 and clathrin as well as APs, while the relative targets detected on cell lyses were performed as loading control. ( C ) Immunofluorescent co-staining of clathrin (red) and TGN46 (green, trans-TGN marker) in spermatids. The Elapor1 cKO spermatids displayed rare co-localization of clathrin and TGN46. The arrow indicates the acrosome. Scale bar = 10 μm. ( D ) The localization of clathrin on pro-AVs analyzed by IEM showed that clathrin localized on the surface of most pro-AVs situated between the Golgi apparatus and the acrosome in normal spermatids, whereas clathrin on the pro-AVs disappeared when Elapor1 deficiency. G, Golgi apparatus; A, Acrosome; pre-A, Preacrosomal granules; red arrows, immunogold labelled clathrin. Scale bars in low-magnification = 200 nm, in high-magnification = 100 nm

    Article Snippet: The unspecific binding sites were blocked with 5% goat serum in PBS and then incubated overnight at 4 °C with the primary antibodies against Elapor1 (1:200 dilution), ACRV1 (1:200 dilution, #14040-1-AP, Proteintech), GM130 (1:1000 dilution, #610822, BD Biosciences), Clathrin (1:200 dilution, #26523-1-AP, Proteintech), and TGN46 (1:200 dilution, #66477-1-Ig, Proteintech), respectively.

    Techniques: Recombinant, Immunoprecipitation, Western Blot, Control, Staining, Marker